EngradeWikis › Lab.

Lab. At home bread mold experiment.



In the third paragraph of your Exploring Creation with Biology textbooks (2nd edition, p. 97), you will find the following directions for beginning to grow your bread mold sample at home.


A word of caution for ALL PENICILLIN ALLERGY STUDENTS AND HOUSEHOLDS: Be sure to use caution while handling, breathing around, and transporting mold specimens. Students are encouraged to wear masks, gloves, and a long sleeve shirt to wear over clothing while handling and observing specimens both during the class (lab) as well as at home. When you transport these specimens to class BE ABSOLUTELY CERTAIN TO KEEP THEM IN A DISPOSABLE, TIGHTLY COVERED SHOE BOX or SIMILAR CONTAINER!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!
Exposure to certain molds can be a severe health hazard for any student/family member with a penicillin related allergy!!!


The "quoted" directions have been included below.

"NOTE: In order to perform Experiment 4.3, you will need to grow some mold on bread, jelly, and/or fruit. You need to start that process now. To do this, take a slice of bread (sprinkle a little water on it), a sample of jelly, and a piece of sliced fruit and set them out in the open.
Putting them outside works best, but you will need to find a place where the birds and other mammals cannot get to them. If you can't find such a place outside, put them somewhere like a garage or shed. They will attract fruit flies, so do not put them in the house! If you live in a very arid (dry) climate (like Arizona), put the samples in a plastic bag with some water. Keep the plastic bag open and on its side. This will keep the samples moist, which is necessary for the growth of molds, but it will also keep them exposed to air. As you read through the first part of this module, the mold should start to grow. By the time you reach Experiment 4.3 (in your reading), at least on of the samples should have mold on it. The experiment has non-microscope components, so you should prepare for it whether or not you have a microscope."


The following directions are for the experiment that will follow the growth of the mold samples. While the bread mold growth will take place at home, the lab experiment will be done in class as follows. I will provide a lab observation sheet in class for you to draw your specimens.


Experiment 4.3 Molds

Supplies:
*Bread, jelly, and/or fruit mold grown earlier (Only one specimen is necessary, but if you observe more than one specimen, you will learn more!)
*Magnifying glass
*Knife
*Needle (or probe from your dissection kit)
*Microscope
*Slides and coverslips
*Water
*Eyedropper

Object: To observe various molds and the differences in how they look both macroscopically and microscopically.

Procedure:
1. Observe the mold or molds that you grew. For each specimen, not the source and describe the mold by color, shape, size and texture (fuzzy, smooth, flat, raised, etc.).
2. Observe each specimen with a magnifying glass. Sketch the magnified image, noting the source of each specimen.
3. Using a knife, needle, or probe, scrape off a section of the mold and place a tiny amount on a slide.
4. Observe the dry specimen on all magnifications.
5. Sketch an example of your observations, noting the source of each specimen.
6. Add a drop of water to the specimen on the slide and then cover it with a coverslip.
7. Observe the specimen again on all magnifications and sketch an example, noting the source.
8. If you were able to observe both a bread mold and a mold from fruit, try to note the differences between the two.
9. Clean up: Wash the slides, coverslips, and eyedropper; dry and put everything away. Wipe the microscope lenses with lens paper. Return the microscope to a safe place. Dispose of all molds.
Close